Dynamin 1 Recombinant Mouse Monoclonal Antibody [A1B1-R]
cat.: HA601230
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: A1B1-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 97 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human Dynamin 1 aa 500-800.
Positive control: Rat brain tissue lysate, mouse brain tissue lysate.
Subcellular location: Cytoskeleton, cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q05193 Human | P39053 Mouse | P21575 Rat
Alternative names: B dynamin D100 DNM 1 DNM DNM1 DYN1_HUMAN Dynamin Dynamin-1 Dynamin1
Images
HA601230_1.jpg Fig1: Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (HA601230) at 1/1,000 dilution.

Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 97 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601230) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.