Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | A1B1-R |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 97 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within human Dynamin 1 aa 500-800. |
Positive control: | Rat brain tissue lysate, mouse brain tissue lysate. |
Subcellular location: | Cytoskeleton, cytoplasm. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q05193 Human | P39053 Mouse | P21575 Rat |
Alternative names: | B dynamin D100 DNM 1 DNM DNM1 DYN1_HUMAN Dynamin Dynamin-1 Dynamin1 |
Fig1:
Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (HA601230) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601230) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |