Thymidine Phosphorylase Recombinant Mouse Monoclonal Antibody [A1A10-R]
cat.: HA601231
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A1A10-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Thymidine Phosphorylase aa 19-239 / 482. |
| Positive control: | THP-1 cell lysate, A549 cell lysate, A431 cell lysate, HeLa cell lysate, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:3,000-1:10,000 |
| Uniprot #: | SwissProt: P19971 Human | Q99N42 Mouse | Q5FVR2 Rat |
| Alternative names: | ECGF 1 ECGF ECGF1 Endothelial cell growth factor 1 Endothelial cell growth factor 1 platelet derived Endothelial cell growth factor, platelet-derived Gliostatin hPD ECGF MEDPS1 MNGIE MTDPS1 PD ECGF PD-ECGF PDECGF PDEGF Platelet derived endothelial cell growth factor Platelet derived endothelial growth factor Platelet-derived endothelial cell growth factor TdRPase Thymidine phosphorylase TP Tymp TYPH_HUMAN |
Images
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Fig1:
Western blot analysis of Thymidine Phosphorylase on different lysates with Mouse anti-Thymidine Phosphorylase antibody (HA601231) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601231) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (HA601231) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601231) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-Thymidine Phosphorylase antibody (HA601231) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601231) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.