CD10 Recombinant Mouse Monoclonal Antibody [A1G3-R]
cat.: HA601246
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: A1G3-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 86 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within human CD10 aa 200-300.
Positive control: Daudi cell lysate, Ramos cell lysate, LNCaP cell lysate, mouse kidney tissue lysate, Raji, Ramos, human renal clear cell carcinoma tissue, human small intestine tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:2,000
1:100
1:200-1:1,000
Uniprot #: SwissProt: P08473 Human | Q61391 Mouse | P07861 Rat
Alternative names: Atriopeptidase CALLA CD10 CD10 antigen Common acute lymphocytic leukemia antigen DKFZp686O16152 EC 3.4.24.11 Enkephalinase EPN Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) Membrane metallo endopeptidase Membrane metallo endopeptidase variant 1 Membrane metallo endopeptidase variant 2 Membrane metalloendopeptidase Membrane metalloendopeptidase neutral endopeptidase enkephalinase Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 Membrane metalloendopeptidase variant 1 Membrane metalloendopeptidase variant 2 MGC126681 MGC126707 MME NEP NEP_HUMAN Neprilysin neprilysin-390 neprilysin-411 Neutral endopeptidase 24.11 Neutral endopeptidase Neutral endopeptidase, membrane-associated SFE Skin fibroblast elastase
Images
HA601246_1.jpg Fig1: Western blot analysis of CD10 on different lysates with Mouse anti-CD10 antibody (HA601246) at 1/2,000 dilution.

Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: Ramos cell lysate (20 µg/Lane)
Lane 3: LNCaP cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse kidney tissue lysate (40 µg/Lane)

Predicted band size: 86 kDa
Observed band size: 100 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601246) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HA601246_2.jpg Fig2: Immunocytochemistry analysis of Raji cells labeling CD10 with Mouse anti-CD10 antibody (HA601246) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD10 antibody (HA601246) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
HA601246_3.jpg Fig3: Immunocytochemistry analysis of Ramos cells labeling CD10 with Mouse anti-CD10 antibody (HA601246) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD10 antibody (HA601246) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
HA601246_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-CD10 antibody (HA601246) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601246) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601246_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-CD10 antibody (HA601246) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601246) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601246_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD10 antibody (HA601246) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601246) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601246_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-CD10 antibody (HA601246) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601246) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601246_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-CD10 antibody (HA601246) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601246) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.