Grp75 Recombinant Mouse Monoclonal Antibody [6-A9-R]
cat.: HA601253
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 6-A9-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Grp75 aa 101-150. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, K-562, human liver tissue. |
| Subcellular location: | Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:100 1:500 |
| Uniprot #: | SwissProt: P38646 Human | P38647 Mouse | P48721 Rat |
| Alternative names: | 75 kDa glucose regulated protein 75 kDa glucose-regulated protein CSA Glucose Regulated Protein Grp 75 GRP-75 GRP75 GRP75_HUMAN Heat shock 70 kDa protein 9 Heat shock 70kD protein 9 heat shock 70kDa protein 9 Heat shock 70kDa protein 9B Heat shock protein 74 kDa A Heat shock protein A Heat shock protein cognate 74 Hsc74 Hsp74 Hsp74a HSPA9 Hspa9a HSPA9B MGC4500 mitochondrial Mortalin 2 Mortalin Mortalin perinuclear Mortalin2 MOT 2 MOT MOT2 Mthsp70 p66 mortalin P66 MOT PBP74 Peptide binding protein 74 Peptide-binding protein 74 Stress 70 protein mitochondrial Stress 70 protein mitochondrial precursor Stress-70 protein |
Images
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Fig1:
Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody (HA601253) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601253) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of K-562 cells labeling Grp75 with Mouse anti-Grp75 antibody (HA601253) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody (HA601253) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Grp75 antibody (HA601253) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601253) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.