MGST1 Recombinant Mouse Monoclonal Antibody [14H2-R]
cat.: HA601255
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 14H2-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MGST1 aa 1-144 / 155. |
| Positive control: | SiHa cell lysate, HepG2 cell lysate, A549 cell lysate, U-937 cell lysate, MCF7 cell lysate, Rat liver tissue lysate, human liver tissue. |
| Subcellular location: | Endoplasmic reticulum, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P10620 Human | P08011 Rat |
| Alternative names: | MGST1 Glutathione S transferase 12 GST12 MGST 1 MGST MGST I MGST1 MGST1_HUMAN Microsomal glutathione S-transferase 1 Microsomal GST 1 Microsomal GST-1 Microsomal GST-I |
Images
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Fig1:
Western blot analysis of MGST1 on different lysates with Mouse anti-MGST1 antibody (HA601255) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: U-937 cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 15 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601255) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MGST1 on different lysates with Mouse anti-MGST1 antibody (HA601255) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 18 kDa Observed band size: 15 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601255) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-MGST1 antibody (HA601255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.