CD42a Recombinant Mouse Monoclonal Antibody [A5E6-R]
cat.: HA601262
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A5E6-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within N-terminal human CD42a. |
| Positive control: | HeLa cell lysate, human lung tissue lysate, mouse spleen tissue lysate, mouse lung tissue lysate, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: P14770 Human | O88186 Mouse |
| Alternative names: | CD42a Glycoprotein 9 Glycoprotein IX Glycoprotein IX (platelet) Glycoprotein IX precursor GP IX GP-IX GP9 GPIX GPIX_HUMAN Platelet glycoprotein IX |
Images
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Fig1:
Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Human lung tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Mouse lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 28 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601262) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.