CD42a Recombinant Mouse Monoclonal Antibody [A5E6-R]
cat.: HA601262
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: A5E6-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG1
Immunogen: Recombinant protein within N-terminal human CD42a.
Positive control: HeLa cell lysate, human lung tissue lysate, mouse spleen tissue lysate, mouse lung tissue lysate, mouse spleen tissue, rat spleen tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:2,000
1:2,000
Uniprot #: SwissProt: P14770 Human | O88186 Mouse
Alternative names: CD42a Glycoprotein 9 Glycoprotein IX Glycoprotein IX (platelet) Glycoprotein IX precursor GP IX GP-IX GP9 GPIX GPIX_HUMAN Platelet glycoprotein IX
Images
HA601262_1.jpg Fig1: Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Human lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Mouse lung tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 19 kDa
Observed band size: 28 kDa

Exposure time: 1 minute 18 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601262) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HA601262_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601262_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.