TROP2 Recombinant Mouse Monoclonal Antibody [A10G7-R]
cat.: HA601263
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: IHC-P, WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: A10G7-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 36 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human TROP2 aa 1-274 / 323 (Extracellular).
Positive control: MCF7 cell lysate, SK-Br-3 cell lysate, A431, human bladder tissue, human bladder carcinoma tissue, human breast cancer tissue, human cervix cancer tissue, human esophagus tissue, human liver tissue, human lung squamous cacner tissue, human skin tissue, MDA-MB-468.
Subcellular location: Membrane.
Recommended Dilutions:
  IHC-P
  WB
  IF-Cell
  FC
1:1,000-1:2,000
1:1,000
1:100
1:1,000
Uniprot #: SwissProt: P09758 Human
Alternative names: Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-2 Cell surface glycoprotein Trop2 Epithelial glycoprotein 1 GA733 1 GA7331 M1S 1 M1S1 Membrane component chromosome 1 surface marker 1 Pancreatic carcinoma marker protein GA733 1 Pancreatic carcinoma marker protein GA733-1 Pancreatic carcinoma marker protein GA7331 TACD 2 TACD2_HUMAN TACSTD 2 Tacstd2 Trop 2 Trop2 Tumor associated calcium signal transducer 2 precursor Tumor-associated calcium signal transducer 2
Images
HA601263_1.jpg Fig1: Western blot analysis of TROP2 on different lysates with Mouse anti-TROP2 antibody (HA601263) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate
Lane 2: SK-Br-3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 36 kDa
Observed band size: 50 kDa

Exposure time: 28 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601263) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HA601263_2.jpg Fig2: Immunocytochemistry analysis of A431 cells labeling TROP2 with Mouse anti-TROP2 antibody (HA601263) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-TROP2 antibody (HA601263) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
HA601263_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human bladder tissue with Mouse anti-TROP2 antibody (HA601263) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Mouse anti-TROP2 antibody (HA601263) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-TROP2 antibody (HA601263) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Mouse anti-TROP2 antibody (HA601263) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Mouse anti-TROP2 antibody (HA601263) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TROP2 antibody (HA601263) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human lung squamous cacner tissue with Mouse anti-TROP2 antibody (HA601263) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-TROP2 antibody (HA601263) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601263) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601263_11.jpg Fig11: Flow cytometric analysis of A431 cells labeling TROP2.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601263, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA601263_12.jpg Fig12: Flow cytometric analysis of MDA-MB-468 cells labeling TROP2.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601263, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.