p47-phox Recombinant Mouse Monoclonal Antibody [A6B10-R]
cat.: HA601264
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A6B10-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human p47-phox aa 51-250. |
| Positive control: | Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HL-60 cell lysate. |
| Subcellular location: | Cytoplasm, cytosol, Membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P14598 Human |
| Alternative names: | 47 kDa autosomal chronic granulomatous disease protein 47 kDa neutrophil oxidase factor NADPH oxidase organizer 2 NCF 47K NCF-1 NCF-47K Ncf1 NCF1_HUMAN Neutrophil cytosol factor 1 Neutrophil cytosolic factor 1 neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) Neutrophil NADPH oxidase factor 1 Nox organizer 2 Nox organizing protein 2 Nox-organizing protein 2 NOXO2 p47 phox p47-phox SH3 and PX domain containing protein 1A SH3 and PX domain-containing protein 1A SH3PXD1A |
Images
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Fig1:
Western blot analysis of p47-phox on different lysates with Mouse anti-p47-phox antibody (HA601264) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Raji cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601264) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.