Glucosidase 2 subunit beta Recombinant Mouse Monoclonal Antibody [A6H9-R]
cat.: HA601276
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A6H9-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 59 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human Glucosidase 2 subunit beta aa 51-250.
Positive control: HeLa cell lysate, 293T cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate.
Subcellular location: Endoplasmic reticulum.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P14314 Human
Alternative names: 80K-H protein AGE-binding receptor 2 AGE-R2 G19P1 GLU2B_HUMAN Glucosidase 2 subunit beta Glucosidase II beta subunit Glucosidase II subunit beta Hepatocystin PCLD PKCSH PLD1 PRKCSH Protein kinase C substrate 60.1 kDa protein heavy chain Protein kinase C substrate 80 Kda protein Protein kinase C substrate 80K-H Protein kinase C substrate, 80 Kda protein
Images
HA601276_1.jpg Fig1: Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA601276) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Daudi cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A431 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 59 kDa
Observed band size: 80 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601276) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.