GOT1 Recombinant Mouse Monoclonal Antibody [A2D6-R]
cat.: HA601279
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A2D6-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human GOT1 aa 16-211 / 413. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, Caco-2 cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse heart tissue lysate, rat liver tissue lysate, rat heart tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P17174 Human | P05201 Mouse | P13221 Rat |
| Alternative names: | AATC_HUMAN Aspartate aminotransferase 1 Aspartate aminotransferase Aspartate aminotransferase cytoplasmic Aspartate aminotransferase cytosolic ASTQTL1 cAspAT cCAT cysteine aminotransferase, cytoplasmic cysteine transaminase, cytoplasmic cytoplasmic ec 2.6.1.1 GIG 18 GIG18 Glutamate oxaloacetate transaminase 1 Glutamate oxaloacetate transaminase soluble Glutamic oxaloacetic transaminase 1 soluble GOT 1 GOT1 Growth inhibiting protein 18 SGOT Transaminase A |
Images
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Fig1:
Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (HA601279) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: HL-60 cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Mouse heart tissue lysate (40 µg/Lane) Lane 8: Rat liver tissue lysate (40 µg/Lane) Lane 9: Rat heart tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 38 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601279) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of GOT1 on different lysates with Mouse anti-GOT1 antibody (HA601279) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GOT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601279) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.