DNMT1 Recombinant Mouse Monoclonal Antibody [A3A8-R]
cat.: HA601281
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3A8-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 183 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Dnmt1 aa 500-600 / 1616. |
| Positive control: | HeLa cell lysate, 293T cell lysate, HepG2 cell lysate, Jurkat cell lysate, HeLa, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100 1:800 |
| Uniprot #: | SwissProt: P26358 Human |
| Alternative names: | ADCADN AIM CXXC finger protein 9 CXXC-type zinc finger protein 9 CXXC9 DNA (cytosine 5 ) methyltransferase 1 DNA (cytosine-5)-methyltransferase 1 DNA methyltransferase 1 DNA methyltransferase HsaI DNA methyltransferase M.HsaI. DNA MTase DNA MTase HsaI DNMT 1 DNMT Dnmt1 DNMT1_HUMAN Dnmt1o FLJ16293 HSN1E M.HsaI MCMT Met1 MGC104992 mMmul MommeD2 |
Images
|
Fig1:
Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (HA601281) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 µg/Lane Lane 2: 293T cell lysate, 20 µg/Lane Lane 3: HepG2 cell lysate, 20 µg/Lane Lane 4: Jurkat cell lysate, 20 µg/Lane Predicted band size: 183 kDa Observed band size: 183 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601281) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling DNMT1 with Mouse anti-DNMT1 antibody (HA601281) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DNMT1 antibody (HA601281) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-DNMT1 antibody (HA601281) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601281) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.