Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R]
cat.: HA601283
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, ELISA, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 54-76-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human IgG heavy chain. |
| Positive control: | Recombinant Human IgG protein, human tonsil tissue. |
| Recommended Dilutions:
WB ELISA IHC-P |
1:1,000 1:1,000-1:20,000 1:200 |
| Uniprot #: | SwissProt: P01857 Human | P01859 Human | P01860 Human | P01861 Human |
| Alternative names: | FLJ39988 FLJ40036 FLJ40253 FLJ40587 FLJ40789 FLJ40834 G1m marker G2m marker G3m marker G4m marker HDC Heavy chain disease protein Human immunglobulin G Ig gamma 1 chain C region Ig gamma 2 chain C region Ig gamma 3 chain C region Ig gamma 4 chain C region IgG heavy chain locus IGHG 1 IGHG 2 IGHG 3 IGHG 4 IGHG1 IGHG2 IGHG3 IGHG4 Immunoglobulin Gm1 Immunoglobulin Gm3 Immunoglobulin Gm4 immunoglobulin heavy constant gamma 1 (G1m marker) Immunoglobulin heavy constant gamma 1 immunoglobulin heavy constant gamma 2 (G2m marker) Immunoglobulin heavy constant gamma 2 immunoglobulin heavy constant gamma 3 (G3m marker) Immunoglobulin heavy constant gamma 3 immunoglobulin heavy constant gamma 4 (G4m marker) Immunoglobulin heavy constant gamma 4 MGC117419 MGC45809 |
Images
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Fig1:
Western blot analysis of Human IgG heavy chain on Recombinant Human IgG protein with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Observed band size: 50 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601283) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.