CD43 Recombinant Mouse Monoclonal Antibody [A2F8-R]
cat.: HA601286
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | A2F8-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human CD43. |
| Positive control: | K-562 cell lysate, HL-60 cell lysate, THP-1 cell lysate, U-937 cell lysate, U-937, human B lymphocyte tumor tissue, human tonsil tissue. |
| Subcellular location: | Membrane, microvillus, uropodium; Nucleus, PML body. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:1,000 1:250 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P16150 Human |
| Alternative names: | CD 43 CD43 CD43 antigen Galactoglycoprotein GALGP GPL 115 GPL115 Human gene for sialophorin Leucocyte sialoglycoprotein LEUK_HUMAN Leukocyte large sialoglycoprotein Leukocyte sialoglycoprotein Leukosialin LSN Ly-48 sialophorin (gpL115, leukosialin, CD43) Sialophorin Spn |
Images
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Fig1:
Western blot analysis of CD43 on different lysates with Mouse anti-CD43 antibody (HA601286) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601286) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43 with Mouse anti-CD43 antibody (HA601286) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43 antibody (HA601286) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43 antibody (HA601286) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601286) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD43 antibody (HA601286) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601286) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Application: IF-Tissue Species: Human Site: tonsil Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
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Fig6:
Application: IF-Tissue Species: Human Site: tonsil Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.