eIF-6 Recombinant Mouse Monoclonal Antibody [A6A2-R]
cat.: HA601294
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A6A2-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human eIF-6 aa 1-200. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus, nucleolus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P56537 Human | O55135 Mouse | Q3KRD8 Rat |
| Alternative names: | b(2)gcn B(2)GCN homolog B4 integrin interactor Binding protein of beta-4 integrin CAB eIF-6 EIF3A EIF6 Eukaryotic translation initiation factor 3A Eukaryotic translation initiation factor 6 IF6_HUMAN Integrin beta 4 binding protein ITGB4BP OK/SW-cl.27 p27 beta 4 integrin binding protein p27(BBP) p27BBP RP4-614O4.1 |
Images
|
Fig1:
Western blot analysis of eIF-6 on different lysates with Mouse anti-eIF-6 antibody (HA601294) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601294) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.