| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A6E8-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human uPA Receptor aa 51-250/335. |
| Positive control: | U937 treated with 200 nM PMA for 72 hours cell lysate. |
| Subcellular location: | Cell membrane, invadopodium membrane; Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q03405 Human |
| Alternative names: | CD 87 CD87 CD87 antigen MO 3 MO3 Monocyte activation antigen Mo3 Plasminogen activator receptor urokinase Plasminogen activator urokinase receptor PLAUR U PAR u plasminogen activator receptor U-PAR u-plasminogen activator receptor form 2 UPA receptor uPAR UPAR_HUMAN Urinary plasminogen activator receptor URKR Urokinase plasminogen activator receptor Urokinase plasminogen activator surface receptor urokinase-type plasminogen activator (uPA) receptor |
|
Fig1:
Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody (HA601296) at 1/1,000 dilution. Lane 1: U937 cell lysate Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-60 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601296) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |