Ephrin A1 Recombinant Mouse Monoclonal Antibody [A7B10-R]
cat.: HA601300
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A7B10-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Ephrin A1 aa 13-190 / 205. |
| Positive control: | HUVEC cell lysates, SK-Br-3 cell lysates. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P20827 Human |
| Alternative names: | B61 ECKLG EFL 1 EFL1 EFNA 1 Efna1 EFNA1_HUMAN EPH related receptor tyrosine kinase ligand 1 EPH-related receptor tyrosine kinase ligand 1 Ephrin-A1 Ephrin-A1, secreted form EphrinA1 EPLG 1 EPLG1 Immediate early response protein B61 LERK 1 LERK-1 LERK1 Ligand of eph related kinase 1 OTTHUMP00000033242 OTTHUMP00000033271 secreted form TNF alpha-induced protein 4 TNFAIP 4 TNFAIP4 Tumor necrosis factor alpha induced protein 4 Tumor necrosis factor alpha-induced protein 4 |
Images
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Fig1:
Western blot analysis of Ephrin A1 on HUVEC cell lysates with Mouse anti-Ephrin A1 antibody (HA601300) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 25 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601300) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Ephrin A1 on SK-Br-3 cell lysates with Mouse anti-Ephrin A1 antibody (HA601300) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601300) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.