Annexin A3 Recombinant Mouse Monoclonal Antibody [A1F7-R]
cat.: HA601303
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A1F7-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Annexin A3 aa 1-323 / 323. |
| Positive control: | A431 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, HT-29 cell lysate, MCF7 cell lysate, human lung tissue lysate, mouse lung tissue lysate. |
| Subcellular location: | Cytosol, extracellular exosome, plasma membrane, axon, cytoplasm, dendrite, membrane, neuronal cell body, phagocytic vesicle membrane, specific granule. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P12429 Human | O35639 Mouse | P14669 Rat |
| Alternative names: | 2-cyclic phosphate 2-phosphohydrolase 35-alpha calcimedin Annexin A3 Annexin III Annexin-3 ANX3 ANXA 3 Anxa3 ANXA3_HUMAN Calcimedin 35 alpha Inositol 1 2 cyclic phosphate 2 phosphohydrolase Inositol 1 Lipocortin III OTTHUMP00000160701 OTTHUMP00000219237 OTTHUMP00000219238 PAP III PAP-III Placental anticoagulant protein III |
Images
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Fig1:
Western blot analysis of Annexin A3 on different lysates with Mouse anti-Annexin A3 antibody (HA601303) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: HT-29 cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: Human lung tissue lysate (40 µg/Lane) Lane 8: Mouse lung tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 33/36 kDa Exposure time: 12 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601303) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.