ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]
cat.: HA601305
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | J0-8-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human ESD aa 1-182 / 282. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse colon tissue lysate, mouse stomach tissue lysate, rat kidney tissue lysate, rat colon tissue lysate, rat stomach tissue lysate. |
| Subcellular location: | Cytoplasm. Cytoplasmic vesicle. |
| Recommended Dilutions:
WB |
1:1,000-1:20,000 |
| Uniprot #: | SwissProt: P10768 Human | Q9R0P3 Mouse | B0BNE5 Rat |
| Alternative names: | EC 3.1.2.12 Es-10 Es10 ESD ESTD_HUMAN Esterase 10 Esterase D Esterase D formylglutathione hydrolase FGH FGH, included FLJ11763 Methylumbelliferyl acetate deacetylase MGC139609 OTTHUMP00000018374 OTTHUMP00000040929 S formylglutathione hydrolase S-formylglutathione hydrolase S-formylglutathione hydrolase, included Sid 478 sid478 |
Images
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Fig1:
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: SW480 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Mouse stomach tissue lysate Lane 5: Rat kidney tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/20,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ESD KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/20,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.