RBM15B Mouse Monoclonal Antibody [A10D12+A10D11]
cat.: HA601308
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A10D12+A10D11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human RBM15B aa 1-200 / 890. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Nucleus, nucleoplasm, Nucleus speckle, Nucleus envelope. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q8NDT2 Human | Q6PHZ5 Mouse Entrez Gene: 315988 Rat |
| Alternative names: | 1810017N16Rik Chromosome 3p21.1 gene sequence HUMAGCGB huOTT3 One twenty two protein 3 One-twenty two protein 3 OTT3 Putative RNA binding protein 15B Putative RNA-binding protein 15B RB15B_HUMAN RBM15B RGD1305937 RNA binding motif protein 15B RNA-binding motif protein 15B |
Images
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Fig1:
Western blot analysis of RBM15B on different lysates with Mouse anti-RBM15B antibody (HA601308) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27602518). The bands below 100 kDa may be caused by degradation. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601308) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.