BrdU Recombinant Mouse Monoclonal Antibody [A9C7-R]
cat.: HA601311
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | IHC-P, IF-Tissue, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A9C7-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | BrdU-OVA |
| Positive control: | BrdU treated mouse embryo tissue, BrdU treated NIH/3T3. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P IF-Tissue IF-Cell FC |
1:5,000 1:500 1:250 1:1,000 |
| Alternative names: | Bromodeoxyuridine BUdr |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded BrdU treated mouse embryo tissue with Mouse anti-BrdU antibody (HA601311) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601311) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded BrdU treated mouse embryo tissue labeling BrdU with Mouse anti-BrdU antibody (HA601311) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601311, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunofluorescence analysis of paraffin-embedded BrdU treated mouse embryo tissue labeling BrdU with Mouse anti-BrdU antibody (HA601311) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601311, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunocytochemistry analysis of BrdU/EdU treated NIH/3T3 cells labeling BrdU with Mouse anti-BrdU antibody (HA601311) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-BrdU antibody (HA601311) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Dot plot showing BrdU treated NIH/3T3 cells stained with HA601311. Cells were incubated with 10 µM BrdU for 30 minutes prior to being harvested, washed twice in 1x PBS and fixed in 70% ethanol at 4℃ for 30 minutes. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature and then neutralised with borate buffer (0.1M, pH8.5) for 15 minutes. Samples were washed and incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (HA601311, 1µg/ml) for 30 min at room temperature. The secondary antibody used was iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) at 1/1,000 dilution for 30 minutes at room temperature. PI was added to cells 15 min prior to data acquisition. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.