Monoamine Oxidase A / MAO-A Recombinant Mouse Monoclonal Antibody [5D1-R]
cat.: HA601319
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 5D1-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MAO-A aa 222-517 / 527. |
| Positive control: | Rat brain tissue lysate, mouse brain tissue lysate, human colon cancer tissue, human liver tissue. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P21397 Human | Q64133 Mouse | P21396 Rat |
| Alternative names: | Amine oxidase [flavin containing] A Amine oxidase [flavin-containing] A AOFA AOFA_HUMAN EC 1.4.3.4 MAO A MAO-A maoA Monoamine oxidase A Monoamine oxidase type A |
Images
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Fig1:
Western blot analysis of Monoamine Oxidase A / MAO-A on different lysates with Mouse anti-Monoamine Oxidase A / MAO-A antibody (HA601319) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 60 kDa Observed band size: 70 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-Monoamine Oxidase A / MAO-A antibody (HA601319) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Monoamine Oxidase A / MAO-A antibody (HA601319) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.