RPS6 Recombinant Mouse Monoclonal Antibody [A6B8-R]
cat.: HA601324
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A6B8-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human RPS6 aa 1-150. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HCT 116 cell lysate, THP-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytosol, Endoplasmic reticulum, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P62753 Human | P62754 Mouse | P62755 Rat |
| Alternative names: | 40S ribosomal protein S6 Air8 NP33 Phosphoprotein NP33 Pp30 Ribosomal protein S6 RP S6 rps6 RS6 RS6_HUMAN S6 S6 Ribosomal Protein |
Images
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Fig1:
Western blot analysis of RPS6 on different lysates with Mouse anti-RPS6 antibody (HA601324) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: HCT 116 cell lysate Lane 6: THP-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.