EIF3H Recombinant Mouse Monoclonal Antibody [PSH04-49]
cat.: HA601328
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human EIF3H aa 1-352 / 352. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, A549 cell lysate, SK-Br-3 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast cancer tissue, human colon cancer tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:200 |
| Uniprot #: | SwissProt: O15372 Human | Q91WK2 Mouse | Q6P9U8 Rat |
| Alternative names: | eIF-3-gamma eIF3 gamma eIF3 p40 eIF3 p40 subunit eIF3h EIF3H_HUMAN EIF3S3 Eukaryotic translation initiation factor 3 subunit 3 Eukaryotic translation initiation factor 3 subunit H MGC102958 |
Images
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Fig1:
Western blot analysis of EIF3H on different lysates with Mouse anti-EIF3H antibody (HA601328) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601328) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3H antibody (HA601328) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EIF3H antibody (HA601328) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.