PD-L2 Mouse Monoclonal Antibody [A10D8]
cat.: HA601331
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A10D8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human PD-L2 aa 1-220 / 273. |
| Positive control: | Saos-2 cell lysate, MG-63 cell lysate, HDLM-2. |
| Subcellular location: | Secreted; Endomembrane system; Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q9BQ51 Human |
| Alternative names: | B7 dendritic cell molecule B7-DC B7DC bA574F11.2 Btdc Butyrophilin B7 DC Butyrophilin B7-DC Butyrophilin B7DC CD 273 CD273 CD273 antigen MGC142238 MGC142240 PD 1 ligand 2 PD L2 PD-1 ligand 2 PD-L2 PD1 ligand 2 PD1L2_HUMAN PDCD 1 ligand 2 PDCD1 ligand 2 PDCD1L2 Pdcd1lg2 PDL 2 PDL2 Programmed cell death 1 ligand 2 Programmed death ligand 2 |
Images
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Fig1:
Western blot analysis of PD-L2 on different lysates with Mouse anti-PD-L2 antibody (HA601331) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate (hot lysis) Lane 2: Jurkat cell lysate (negative) Lane 3: MG-63 cell lysate (hot lysis) Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 50 kDa Exposure time: Lane 1-2: 1 minute; Lane 3: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601331) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HDLM-2 (positive) and Jurkat (negative) labeling PD-L2 with Mouse anti-PD-L2 antibody (HA601331) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PD-L2 antibody (HA601331) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.