Human SAA1 Mouse Monoclonal Antibody [A10E7]
cat.: HA601333
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | A10E7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 13.5 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human SAA1 aa 19-122 (P0DJI8). |
| Positive control: | Recombinant human SAA protein (HA210927). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [A10E6] to Human SAA1 (Detector) (HA601334) and recombinant standard Human SAA1 (HA210927). The reference range value is 329-80,000 pg/ml. |
| Uniprot #: | SwissProt: P0DJI8 Human |
| Alternative names: | amyloid A, serum Amyloid fibril protein AA Amyloid protein A MGC111216 PIG4 SAA SAA2 Serum amyloid A protein serum amyloid A-1 protein serum amyloid A1 TP53I4 Tumor protein p53 inducible protein 4 |
Images
|
Fig1:
Sandwich ELISA analysis of Human SAA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601333) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human SAA protein (HA210927) starting from 80000 pg/ml to 0 pg/ml and detect antibody (HA601334, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2: The concentrations of SAA were measured in duplicates, interpolated from the SAA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.