Recombinant Mouse IgG1 Recombinant Mouse Monoclonal Antibody [PSH04-91]
cat.: HA601336
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Applications: | WB, FC, IP, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PSH04-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Small molecule. |
| Recommended Dilutions:
WB FC IP ChIP |
1:1,000 1:1,000 1-2μg/sample Use 0.5~2 μg for 25 μg of chromatin. |
Images
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Fig1:
PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601172 IP in HeLa cell lysate Lane 3: Recombinant Mouse IgG1 (HA601336) instead of HA601172 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 59 seconds; ECL: K1801 |
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Fig2:
PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA601172 IP in NIH/3T3 cell lysate Lane 3: Recombinant Mouse IgG1 (HA601336) instead of HA601172 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds; ECL: K1802 |
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Fig3:
PCNA was immunoprecipitated from 0.2 mg PC-12 cell lysate with HA601172 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PC-12 cell lysate (input) Lane 2: HA601172 IP in PC-12 cell lysate Lane 3: Recombinant Mouse IgG1 (HA601336) instead of HA601172 in PC-12 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL: K1801 |
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Fig4:
Flow cytometric analysis of HeLa cells labeling eIF-6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601293, 1μg/mL) (green) compared with Recombinant Mouse IgG1 (HA601336, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of C6 cells labeling eIF-6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601293, 1μg/mL) (green) compared with Recombinant Mouse IgG1 (HA601336, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H3 (acetyl K27) (HA600047) or Recombinant Mouse IgG1 (HA601336) according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.