GFAP Recombinant Mouse Monoclonal Antibody [PSH05-17]
cat.: HA601342
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH05-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human GFAP. |
| Positive control: | Recombinant human GFAP protein (HA210990). |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH05-16] to GFAP (Capture) (HA722234) and recombinant Human GFAP protein (HA210990) as the standard. The reference range value is 823-200,000pg/ml. |
| Uniprot #: | SwissProt: P14136 Human | P03995 Mouse | P47819 Rat |
| Alternative names: | wu:fb34h11 ALXDRD cb345 etID36982.3 FLJ42474 FLJ45472 GFAP GFAP_HUMAN gfapl Glial fibrillary acidic protein Intermediate filament protein wu:fk42c12 xx:af506734 zgc:110485 |
Images
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Fig1:
Sandwich ELISA analysis of Human GFAP matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722234) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human GFAP protein (HA210990) starting from 100000 pg/ml to 0 pg/ml and detect antibody (HA601342, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: The concentrations of GFAP were interpolated from the GFAP standard curve and corrected for sample dilution. The mean GFAP concentration was determined to be 41.29 ng/ml in rat brain tissue extract. There was no detectable signal in 293T and NIH/3T3 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.