Kindlin 2 Mouse Monoclonal Antibody [A10G12]
cat.: HA601343
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | A10G12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within human Kindlin 2 aa 301-350 / 680. |
| Positive control: | MDA-MB-231 cell lysate, Saos-2 cell lysate, mouse heart tissue lysate, mouse lung tissue lysate, rat heart tissue lysate, rat lung tissue lysate, MDA-MB-231. |
| Subcellular location: | Cytoplasm, Nucleus, Cell surface. |
| Recommended Dilutions:
WB IF-Cell IP |
1:1,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q96AC1 Human | Q8CIB5 Mouse Entrez Gene: 289992 Rat |
| Alternative names: | FLJ34213 FLJ44462 UNC112 FERM2_HUMAN Fermitin family homolog 2 Fermt2 KIND2 Kindlin-2 MIG-2 MIG2 Mitogen-inducible gene 2 protein PH domain-containing family C member 1 Pleckstrin homology domain containing family C (with FERM domain) member 1 Pleckstrin homology domain-containing family C member 1 PLEKHC1 UNC112B |
Images
|
Fig1:
Western blot analysis of Kindlin 2 on different lysates with Mouse anti-Kindlin 2 antibody (HA601343) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: Saos-2 cell lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (40 µg/Lane) Lane 4: Mouse lung tissue lysate (40 µg/Lane) Lane 5: Rat heart tissue lysate (40 µg/Lane) Lane 6: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 78 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601343) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of MDA-MB-231 cells labeling Kindlin 2 with Mouse anti-Kindlin 2 antibody (HA601343) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Kindlin 2 antibody (HA601343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Kindlin 2 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA601343 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA601343 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MDA-MB-231 cell lysate (input) Lane 2: HA601343 IP in MDA-MB-231 cell lysate Lane 3: Mouse IgG instead of HA601343 in MDA-MB-231 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 38 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.