CCK4 Mouse Monoclonal Antibody [A11A9]
cat.: HA601344
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A11A9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 118 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human CCK4 aa 31-704 / 1,070. |
| Positive control: | HeLa cell lysate, Caco-2 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, NIH/3T3, PC-12. |
| Subcellular location: | Membrane, Cell junction. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:500 |
| Uniprot #: | SwissProt: Q13308 Human | Q8BKG3 Mouse Entrez Gene: 301242 Rat |
| Alternative names: | 8430404F20Rik CCK 4 CCK-4 Colon carcinoma kinase 4 Human colon carcinoma kinase 4 (CCK4) mRNA complete cds Inactive tyrosine-protein kinase 7 mPTK7/CCK4 OTTHUMP00000217016 Protein tyrosine kinase PTK 7 Protein-tyrosine kinase 7 Pseudo tyrosine kinase receptor 7 PTK 7 PTK7 PTK7 protein tyrosine kinase 7 PTK7 protein tyrosine kinase PTK7_HUMAN Serum response factor Srf Tyrosine-protein kinase-like 7 |
Images
|
Fig1:
Western blot analysis of CCK4 on different lysates with Mouse anti-CCK4 antibody (HA601344) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate (negative) Lane 3: Caco-2 cell lysate Lane 4: Jurkat cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lane 9: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 118 kDa Observed band size: 150 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601344) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa cells labeling CCK4 with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling CCK4 with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
|
Fig4:
Immunocytochemistry analysis of PC-12 cells labeling CCK4 with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CCK4 antibody (HA601344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.