BRG1 Recombinant Mouse Monoclonal Antibody [A5C9-R]
cat.: HA601345
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A5C9-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 185 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human BRG1 aa 230-280. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C6 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P51532 Human | Q3TKT4 Mouse | Q8K1P7 Rat |
| Alternative names: | ATP dependent helicase SMARCA4 ATP-dependent helicase SMARCA4 BAF 190 BAF190 BAF190A Brahma protein like 1 BRG1 BRG1 associated factor 190A BRG1 protein BRG1-associated factor 190A BRM/SWI2 related gene 1 Global transcription activator homologous sequence global transcription activator snf2l4 Homeotic gene regulator hSNF2b Mitotic growth and transcription activator MRD16 Nuclear protein GRB1 Protein brahma homolog 1 Protein BRG-1 Protein BRG1 RTPS2 SMARC A4 SMARCA4 SMCA4_HUMAN SNF2 SNF2 beta SNF2 like 4 SNF2-beta SNF2B SNF2L4 SNF2LB Sucrose nonfermenting like 4 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 SWI2 Transcription activator BRG1 |
Images
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Fig1:
Western blot analysis of BRG1 on different lysates with Mouse anti-BRG1 antibody (HA601345) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: 293T cell lysate Lane 4: A549 cell lysate (negative) Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 185 kDa Observed band size: 200 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601345) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.