COMP Mouse Monoclonal Antibody [A10H2]
cat.: HA601354
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A10H2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human COMP aa 321-370. |
| Positive control: | Mouse articular cartilage tissue lysate, Rat articular cartilage tissue lysate, HCT 116 cell lysate, mouse cartilage tissue, rat cartilage tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:400 |
| Uniprot #: | SwissProt: P49747 Human | Q9R0G6 Mouse | P35444 Rat |
| Alternative names: | cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) Cartilage oligomeric matrix protein Cartilage oligomeric matrix protein precursor COMP COMP_HUMAN EDM 1 EDM1 EPD 1 EPD1 Epiphyseal dysplasia 1 Epiphyseal dysplasia 1 multiple Epiphyseal dysplasia multiple 1 MED MGC13181 MGC149768 PSACH pseudoachondroplasia (epiphyseal dysplasia 1, multiple) Pseudoachondroplasia THBS 5 THBS5 Thrombospondin 5 Thrombospondin-5 Thrombospondin5 TSP5 |
Images
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Fig1:
Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution. Lane 1: Mouse articular cartilage tissue lysate (40 µg/Lane) Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: Rat articular cartilage tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 µg/Lane) Lane 2: HCT 116 cell lysate (30 µg/Lane) Predicted band size: 83 kDa Observed band size: 100 kDa Exposure time: 3 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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