Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)
cat.: HA601367
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, mIHC |
| Clone number: | JM36-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Immunogen: | Synthetic peptide within N-terminal human Iba1. |
| Positive control: | Mouse brain tissue, mouse striatum tissue, rat striatum tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup. |
| Recommended Dilutions:
IHC-Fr IHC-P mIHC |
1:500-1:1,000 1:100-1:500 1:500 |
| Uniprot #: | SwissProt: P55008 Human | O70200 Mouse | P55009 Rat |
| Alternative names: | AIF 1 AIF-1 Aif1 AIF1 protein AIF1_HUMAN Allograft inflammatory factor 1 Allograft inflammatory factor 1 splice variant G allograft inflammatory factor-1 splice variant Hara-1 balloon angioplasty responsive transcription BART 1 G1 G1 putative splice variant of allograft inflamatory factor 1 IBA 1 IBA1 interferon gamma responsive transcript Interferon responsive transcript 1 interferon responsive transcript factor 1 Ionized calcium binding adapter molecule 1 Ionized calcium-binding adapter molecule 1 ionized calcium-binding adapter molecule IRT 1 IRT1 Microglia response factor MRF1 Protein g1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 (Iba1, HA601367, green); 1/1,000 (NeuN, ET1602-12, red) Antigen retrieval: Not required |
|
Fig3:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA601367, green); 1/1,000 (GLAST /EAAT1, ET1704-54, red) Antigen retrieval: Not required |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Iba1 antibody (HA601367) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Iba1 antibody (HA601367) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Iba1 antibody (HA601367) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601367) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.