Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)
cat.: HA601367
Product Type: Recombinant Chimeric Antibody, primary antibodies
Species reactivity: Mouse, Rat, Cynomolgus monkey, Pig
Applications: IHC-Fr, IHC-P, mIHC, IF-Cell
Clone number: JM36-62
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Immunogen: Synthetic peptide within N-terminal human Iba1.
Positive control: Mouse brain tissue, mouse striatum tissue, rat striatum tissue.
Subcellular location: Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.
Recommended Dilutions:
  IHC-Fr
  IHC-P
  mIHC
  IF-Cell
1:500-1:1,000
1:100-1:500
1:500
1:100
Uniprot #: SwissProt: P55008 Human | O70200 Mouse | P55009 Rat
Alternative names: AIF 1 AIF-1 Aif1 AIF1 protein AIF1_HUMAN Allograft inflammatory factor 1 Allograft inflammatory factor 1 splice variant G allograft inflammatory factor-1 splice variant Hara-1 balloon angioplasty responsive transcription BART 1 G1 G1 putative splice variant of allograft inflamatory factor 1 IBA 1 IBA1 interferon gamma responsive transcript Interferon responsive transcript 1 interferon responsive transcript factor 1 Ionized calcium binding adapter molecule 1 Ionized calcium-binding adapter molecule 1 ionized calcium-binding adapter molecule IRT 1 IRT1 Microglia response factor MRF1 Protein g1
Images
HA601367_1.jpg Fig1: Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Cerebral cortex
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601367, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.
HA601367_2.jpg Fig2: Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Cerebellum
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: Iba1, 1/1,000 (HA601367, rat, Green), NeuN, 1:1,000 (ET1602-12, rabbit, Red), overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), Goat anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.
HA601367_3.jpg Fig3: Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Cerebellum
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: Iba1, 1/500 (HA601367, rat, Green), GLAST /EAAT1, 1:1,000 (ET1704-54, rabbit, Red), overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), Goat anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.
HA601367_4.jpg Fig4: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Cerebral cortex
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601367, 1/500, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
HA601367_5.jpg Fig5: Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Striatum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601367, 1/100, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
HA601367_6.jpg Fig6: Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Striatum
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601367, 1/200, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
HA601367_7.jpg Fig7: Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: BV2 (Mouse microglial cells)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601367, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
HA601367_8.jpg Fig8: Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: RAW264.7 (Mouse monocytic macrophage leukemia cells)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601367, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.

Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.