Iba1 Recombinant Antibody [JM36-62] - Guinea pig IgG2 (Chimeric)
cat.: HA601368
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, WB, IF-Tissue |
| Clone number: | JM36-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Immunogen: | Synthetic peptide within N-terminal human Iba1. |
| Positive control: | Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, THP-1 cell lysates. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup. |
| Recommended Dilutions:
IHC-Fr WB IF-Tissue |
1:200-1:500 1:1,000 1:500 |
| Uniprot #: | SwissProt: P55008 Human | O70200 Mouse | P55009 Rat |
| Alternative names: | AIF 1 AIF-1 Aif1 AIF1 protein AIF1_HUMAN Allograft inflammatory factor 1 Allograft inflammatory factor 1 splice variant G allograft inflammatory factor-1 splice variant Hara-1 balloon angioplasty responsive transcription BART 1 G1 G1 putative splice variant of allograft inflamatory factor 1 IBA 1 IBA1 interferon gamma responsive transcript Interferon responsive transcript 1 interferon responsive transcript factor 1 Ionized calcium binding adapter molecule 1 Ionized calcium-binding adapter molecule 1 ionized calcium-binding adapter molecule IRT 1 IRT1 Microglia response factor MRF1 Protein g1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA601368, red); 1/500 (NeuN, HA601111, green) Antigen retrieval: Not required |
|
Fig3:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
|
Fig5:
Western blot analysis of Iba1 on THP-1 cell lysates with Guinea pig anti-Iba1 antibody (HA601368) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 17 kDa Observed band size: 12 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601368) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig6:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601368, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.