GAD67 Recombinant Mouse Monoclonal Antibody [PSH08-30]
cat.: HA601370
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH08-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human GAD67 aa 1-594. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, Plasma Membrane. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:10,000 1:500-1:2,000 1:500 1:500 |
| Uniprot #: | SwissProt: P48318 Mouse | P18088 Rat |
| Alternative names: | 67 kDa glutamic acid decarboxylase CPSQ1 DCE1 DCE1_HUMAN EC 4.1.1.15 FLJ45882 GAD 67 GAD GAD-67 GAD1 Glutamate decarboxylase 1 (brain, 67kDa) Glutamate decarboxylase 1 Glutamate decarboxylase 1 brain 67kD Glutamate decarboxylase 1 brain 67kDa Glutamate decarboxylase 67 kDa isoform Glutamate decarboxylase, brain, 67-KD OTTHUMP00000041055 SCP |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-GAD67 antibody (HA601370) at 1/500 dilution. Important Notice: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601370, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of frozen rat cerebellum tissue with Mouse anti-GAD67 antibody (HA601370) at 1/500 dilution. Important Notice: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601370, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Western blot analysis of GAD67 on different lysates with Mouse anti-GAD67 antibody (HA601370) at 1/10,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601370) at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD67 antibody (HA601370) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601370) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GAD67 antibody (HA601370) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601370) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-GAD67 antibody (HA601370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-GAD67 antibody (HA601370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Mouse anti-GAD67 antibody (HA601370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Mouse anti-GAD67 antibody (HA601370) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: GAD67, 1/500 (HA601370, Mouse, Green), VGLUT1, 1:500 (HA601449, Guinea pig, Red), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. |
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Fig11:
Application: Immunofluorescence (IF-tissue) Species: Rat Tissue: Hippocampus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: GAD67, 1/500 (HA601370, Mouse, Green), VGLUT1, 1:500 (HA601449, Guinea pig, Red), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), Goat anti-Guinea pig IgG (iFluor™ 594, HA1143), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.