VGLUT1 Recombinant Mouse Monoclonal Antibody [PSH08-34]
cat.: HA601371
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH08-34 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within rat VGluT1 aa 470-560. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate, mouse striatum tissue, mouse cerebellum tissue, rat striatum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500 |
| Uniprot #: | SwissProt: Q3TXX4 Mouse | Q62634 Rat |
| Alternative names: | BNPI Brain specific Na (+) dependent inorganic phosphate cotransporter Brain specific Na dependent inorganic phosphate cotransporter Brain-specific Na(+)-dependent inorganic phosphate cotransporter Slc17a7 Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 Solute carrier family 17 member 7 Vesicular glutamate transporter 1 VGLU1_HUMAN VGLUT 1 VGluT1 |
Images
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Fig1:
Western blot analysis of VGLUT1 on different lysates with Mouse anti-VGLUT1 antibody (HA601371) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 2: Rat brain tissue lysate (no heat) (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.