c-Fos Recombinant Antibody [PSH05-77] - Guinea pig IgG2 (Chimeric)
cat.: HA601374
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, WB |
| Clone number: | PSH05-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Immunogen: | Recombinant protein within human Protein c-Fos aa 1-380. |
| Positive control: | Mouse brain tissue, rat brain tissue. |
| Subcellular location: | Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol. |
| Recommended Dilutions:
IHC-Fr WB |
1:2,000-1:5,000 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P01100 Human | P01101 Mouse | P12841 Rat |
| Alternative names: | Activator protein 1 AP 1 C FOS Cellular oncogene c fos Cellular oncogene fos FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) FBJ murine osteosarcoma viral oncogene homolog FBJ murine osteosarcoma viral v fos oncogene homolog FBJ Osteosarcoma Virus FOS FOS protein FOS_HUMAN G0 G1 switch regulatory protein 7 G0/G1 switch regulatory protein 7 G0S7 Oncogene FOS p55 proto oncogene c Fos Proto oncogene protein c fos Proto-oncogene c-Fos v fos FBJ murine osteosarcoma viral oncogene homolog |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA601374, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer. |
|
Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA601374, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer. |
|
Fig3:
Western blot analysis of c-Fos on different lysates with Guinea pig anti-c-Fos antibody (HA601374) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41-55 kDa Exposure time: 6 seconds; ECL: K1801; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601374) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/10,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.