QKI-5 Recombinant Mouse Monoclonal Antibody [PSH10-22]
cat.: HA601378
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH10-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human QKI-5 aa 301-341 / 341. |
| Positive control: | K-562 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IP |
1:2,000 1:5,000-1:20,000 1:500 1-2μg/sample |
| Uniprot #: | SwissProt: Q96PU8-1 Human | Q9QYS9 Mouse | Q91XU1 Rat |
| Alternative names: | DKFZp586I0923 HKQ Homolog of mouse quaking QKI KH domain RNA binding protein Hqk HQK1 HqkI OTTHUMP00000017581 OTTHUMP00000017582 OTTHUMP00000017583 Protein quaking QK QK1 QK3 QKI QKI_HUMAN QKI1 Quaking homolog Quaking homolog KH domain RNA binding Quaking homolog KH domain RNA binding mouse Quaking isoform 1 Quaking protein RNA binding protein HQK QKI-5 |
Images
|
Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
|
Fig2:
Western blot analysis of QKI-5 on different lysates with Mouse anti-QKI-5 antibody (HA601378) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Indirect ELISA analysis of QKI-5 on different peptides. |
|
Fig6:
QKI-5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601378 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601378 at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601378 IP in HeLa cell lysate Lane 3: Mouse IgG instead of HA601378 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801 |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.