SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]
cat.: HA601384
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH10-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG1 |
| Positive control: | Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasmic vesicle membrane, Presynapse. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:1,000 |
| Uniprot #: | SwissProt: O35633 Mouse | O35458 Rat |
| Alternative names: | bA122O1.1 GABA and glycine transporter hVIAAT SLC32A 1 Slc32a1 solute carrier family 32 (GABA vesicular transporter) member 1 Solute carrier family 32 member 1 Vesicular GABA Amino Acid Transporter Vesicular GABA transporter Vesicular inhibitory amino acid transporter VGAT VIAAT VIAAT_HUMAN |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 (SLC32A1 / VGAT, HA601384, green); 1/500 (VGLUT1, HA723217, red) Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 (SLC32A1 / VGAT, HA601384, green); 1/500 (VGLUT2, HA723218, red) Antigen retrieval: Not required |
|
Fig3:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 (SLC32A1 / VGAT, HA601384, green); 1:500 (Parvalbumin, ET1703-15, red) Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 (SLC32A1 / VGAT, HA601384, green); 1:500 (Parvalbumin, ET1703-15, red) Antigen retrieval: Not required |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig8:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.