Mouse CD45R/B220 Recombinant Rat Monoclonal Antibody [PSH10-80]
cat.: HA601385
| Product Type: | Recombinant Rat Monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | IHC-P, FC, IF-Tissue |
| Clone number: | PSH10-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 145 kDa |
| Isotype: | IgG1 |
| Positive control: | Mouse spleen tissue, mouse spleen cells. |
| Subcellular location: | Cell membrane, Membrane raft, Synapse. |
| Recommended Dilutions:
IHC-P FC IF-Tissue |
1:1,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P06800 Mouse |
| Alternative names: | B220 CD45 CD45 antigen Glycoprotein GP180 L CA L-CA LCA Leukocyte common antigen Leukocyte common antigen precursor loc Ly-5 LY5 Lyt-4 Protein tyrosine phosphatase receptor type C Protein tyrosine phosphatase receptor type c polypeptide Ptprc PTPRC_HUMAN Receptor type tyrosine protein phosphatase C Receptor-type tyrosine-protein phosphatase C T200 T200 glycoprotein T200 leukocyte common antigen |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-Mouse CD45R/B220 antibody (HA601385) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601385) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Flow cytometric analysis of mouse spleen cells labeling Mouse CD45R/B220 (HA601385). Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601385, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Dylight 488 conjugate-Goat anti-Rat IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. |
|
Fig3:
Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.