QKI-7 Recombinant Mouse Monoclonal Antibody [PSH10-92]
cat.: HA601387
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Mouse, Rat, Cynomolgus monkey, Pig
Applications: WB
Clonality: Monoclonal
Clone number: PSH10-92
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within human QKI-7 aa 291-317 / 317.
Positive control: Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: Q9QYS9 Mouse | Q91XU1 Rat
Alternative names: DKFZp586I0923 HKQ Homolog of mouse quaking QKI KH domain RNA binding protein Hqk HQK1 HqkI OTTHUMP00000017581 OTTHUMP00000017582 OTTHUMP00000017583 Protein quaking QK QK1 QK3 QKI QKI_HUMAN QKI1 Quaking homolog Quaking homolog KH domain RNA binding Quaking homolog KH domain RNA binding mouse Quaking isoform 1 Quaking protein RNA binding protein HQK
Images
HA601387_1.jpg Fig1: Western blot analysis of QKI-7 on different lysates with Mouse anti-QKI-7 antibody (HA601387) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Rat cerebellum tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 40 kDa

Exposure time: 21 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HA601387_2.jpg Fig2: Indirect ELISA analysis of QKI-7 on different peptides.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.