P2Y12 Recombinant Antibody [PSH04-48] - Rat IgG1 (Chimeric)
cat.: HA601388
| Product Type: | Recombinant Chimeric Antibody IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | PSH04-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide. |
| Positive control: | Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q9CPV9 Mouse | Q9EPX4 Rat |
| Alternative names: | ADP glucose receptor ADP-glucose receptor ADPG R ADPG-R ADPGR BDPLT8 G protein coupled receptor SP1999 Gi coupled ADP receptor HORK 3 Gi coupled ADP receptor HORK3 HORK 3 HORK3 P2RY 12 P2RY12 P2T(AC) P2Y 12 P2Y purinoceptor 12 P2Y(12)R P2Y(AC) P2Y(ADP) P2Y(cyc) P2Y12 P2Y12 platelet ADP receptor P2Y12_HUMAN Platelet ADP receptor Purinergic receptor P2RY12 Purinergic receptor P2Y G protein coupled 12 Purinergic receptor P2Y12 Putative G protein coupled receptor SP 1999 SP1999 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1: 2,000 (P2Y12, HA601388, green); 1:2,000 (NeuN, ET1602-12, red) Antigen retrieval: Not required |
|
Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1: 2,000 (P2Y12, HA601388, green); 1:2,000 (NeuN, ET1602-12, red) Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-P2Y12 antibody (HA601388) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601388) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-P2Y12 antibody (HA601388) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601388) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-P2Y12 antibody (HA601388) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601388) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-P2Y12 antibody (HA601388) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601388) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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