GAD65 Recombinant Antibody [PSH06-80] - Rat IgG1 (Chimeric)
cat.: HA601389
| Product Type: | Recombinant Chimeric Antibody IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH06-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein. |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Cytoplasmic vesicle, Presynaptic cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:2,000 1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P48320 Mouse | Q05683 Rat |
| Alternative names: | 65 kDa glutamic acid decarboxylase DCE 2 DCE2 DCE2_HUMAN GAD 2 GAD 65 GAD-2 GAD-65 Gad2 Glutamate decarboxylase 2 (pancreas) Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) Glutamate decarboxylase 2 Glutamate Decarboxylase 65 Glutamate decarboxylase 65 kDa isoform Glutamic Acid Decarboxylase 2 Glutamic Acid Decarboxylase 65 MGC161605 MGC161607 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 2,000 (GAD65, HA601389, green); 1:2,000 (EAAT1, ET1704-54, red) Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 2,000 (GAD65, HA601389, green); 1:2,000 (EAAT1, ET1704-54, red) Antigen retrieval: Not required |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-GAD65 antibody (HA601389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of GAD65 on different lysates with Rat anti-GAD65 antibody (HA601389) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 60 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601389) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.