Apolipoprotein E Recombinant Antibody [PSH10-52] - Rat IgG1 (Chimeric)
cat.: HA601391
| Product Type: | Recombinant Chimeric Antibody IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH10-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Mouse Apolipoprotein E aa 19-311. |
| Positive control: | Mouse brain tissue, rat brain tissue, rat striatum tissue, Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:2,000 1:5,000 |
| Uniprot #: | SwissProt: P08226 Mouse | P02650 Rat |
| Alternative names: | AD2 Apo-E APOE APOE_HUMAN APOEA Apolipoprotein E Apolipoprotein E3 ApolipoproteinE Apoprotein LDLCQ5 LPG |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse brain tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601391, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601391) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601391) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Apolipoprotein E antibody (HA601391) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601391) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of Apolipoprotein E on different lysates with Rat anti-Apolipoprotein E antibody (HA601391) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: C2C12 cell lysate (negative) Lane 4: Rat brain tissue lysate Lane 5: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601391) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.