Otx2 Recombinant Antibody [PSH01-61] - Rat IgG1 (Chimeric)
cat.: HA601399
| Product Type: | Recombinant Chimeric Antibody IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P |
| Clone number: | PSH01-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Otx2 aa 1-289. |
| Positive control: | Mouse embryo tissue, rat embryo tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-Fr IHC-P |
1:500 1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P32243 Human | P80206 Mouse | Q64201 Rat |
| Alternative names: | CPHD6 Homeobox protein OTX2 MCOPS 5 MCOPS5 MGC45000 Orthodenticle 2 Orthodenticle homeobox 2 Orthodenticle homolog 2 (Drosophila) Orthodenticle homolog 2 Orthodenticle2 Otx 2 otx2 OTX2_HUMAN |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: E14.5 embryo Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rat anti-Otx2 antibody (HA601399) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601399) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rat anti-Otx2 antibody (HA601399) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601399) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rat anti-Otx2 antibody (HA601399) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601399) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.