Acetylcholinesterase Recombinant Antibody [PSH10-55] - Rat IgG1 (Chimeric)
cat.: HA601400
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr |
| Clone number: | PSH10-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Immunogen: | Recombinant protein within Mouse ACHE aa 32-614. |
| Positive control: | Mouse striatum tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Synapse, Secreted, Cell membrane. |
| Recommended Dilutions:
WB IHC-Fr |
1:5,000 1:500 |
| Uniprot #: | SwissProt: P21836 Mouse | P37136 Rat |
| Alternative names: | ACEE ACES_HUMAN Acetylcholinesterase AChE Apoptosis related acetylcholinesterase ARACHE N ACHE N-ACHE YT YT blood group |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. |
|
Fig2:
Application: IHC-Fr Species: Rat Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. |
|
Fig3:
Western blot analysis of Acetylcholinesterase on different lysates with Rat anti-Acetylcholinesterase antibody (HA601400) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat liver tissue lysate (negative) (20 µg/Lane) Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601400) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.