Peripherin Recombinant Antibody [PSH10-70] - Rat IgG1 (Chimeric)
cat.: HA601405
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, WB, IF-Cell |
| Clone number: | PSH10-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Immunogen: | Recombinant protein within Human aa 1-470. |
| Positive control: | Human colon tissue, mouse colon tissue, rat colon tissue, Neuro-2a cell lysate, Mouse dorsal root ganglion tissue lysate, Neuro-2a. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, axon, Perikaryon. |
| Recommended Dilutions:
IHC-Fr IHC-P WB IF-Cell |
1:500 1:500 1:5,000 1:500 |
| Uniprot #: | SwissProt: P41219 Human | P15331 Mouse | P21807 Rat |
| Alternative names: | NEF 4 NEF4 Neurofilament 4 (57kD) Neurofilament 4 Perf PERI_HUMAN Peripherin PRPH 1 prph PRPH1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rat anti-Peripherin antibody (HA601405) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601405) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rat anti-Peripherin antibody (HA601405) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601405) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rat anti-Peripherin antibody (HA601405) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601405) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of Peripherin on different lysates with Rat anti-Peripherin antibody (HA601405) at 1/5,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: Mouse dorsal root ganglion tissue lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 56 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601405) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
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Fig6:
Immunocytochemistry analysis of Neuro-2a cells labeling Peripherin with Rat anti-Peripherin antibody (HA601405) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Peripherin antibody (HA601405) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.