NF-M Recombinant Antibody [JM11-20] - Rat IgG1 (Chimeric)
cat.: HA601408
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | JM11-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Immunogen: | Synthetic peptide within Human NEFM aa 651-692 / 916. |
| Positive control: | Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:500 1:5,000-1:10,000 |
| Uniprot #: | SwissProt: P07197 Human | P08553 Mouse | P12839 Rat |
| Alternative names: | 150kDa medium NEF3 NEFM Neurofilament 3 NF160 NFM |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-NF-M antibody (HA601408) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601408) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-NF-M antibody (HA601408) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601408) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-NF-M antibody (HA601408) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601408) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Western blot analysis of NF-M on different lysates with Rat anti-NF-M antibody (HA601408) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 102 kDa Observed band size: 140 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601408) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.