BLBP Recombinant Antibody [PSH10-68] - Rat IgG1 (Chimeric)
cat.: HA601411
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, WB |
| Clone number: | PSH10-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Immunogen: | Recombinant protein within Mouse BLBP aa 1-132. |
| Positive control: | Mouse cerebellum tissue, mouse P7 brain tissue, Mouse cerebellum (P0) tissue lysate, Mouse cerebellum (P7) tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IHC-Fr IHC-P WB |
1:500 1:200-1:500 1:5,000 |
| Uniprot #: | SwissProt: P51880 Mouse | P55051 Rat |
| Alternative names: | B FABP B-FABP BFABP BLBP Brain lipid binding protein Brain lipid-binding protein Brain-type fatty acid-binding protein DKFZp547J2313 FABP 7 FABP7 FABP7_HUMAN FABPB Fatty Acid Binding Protein 7 Fatty acid binding protein 7 brain Fatty acid binding protein brain Fatty acid-binding protein 7 Fatty acid-binding protein, brain Mammary derived growth inhibitor related Mammary-derived growth inhibitor related MRG OTTHUMP00000017119 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-BLBP antibody (HA601411) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601411) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse P7 brain tissue with Rat anti-BLBP antibody (HA601411) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601411) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of BLBP on different lysates with Rat anti-BLBP antibody (HA601411) at 1/5,000 dilution. Lane 1: Mouse cerebellum (P0) tissue lysate Lane 2: Mouse cerebellum (P7) tissue lysate Lane 3: Mouse kidney tissue lysate (negative) Lane 4: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: Lane 1-3: 2 seconds; Lane 4: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601411) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.