SOX10 Recombinant Antibody [PDH0-03] - Rat IgG1 (Chimeric)
cat.: HA601413
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, IF-Tissue |
| Clone number: | PDH0-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Immunogen: | Synthetic peptide within C terminal human SOX10. |
| Positive control: | Mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion outer membrane. |
| Recommended Dilutions:
IHC-Fr IHC-P IF-Tissue |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q04888 Mouse | O55170 Rat |
| Alternative names: | DOM DOM Dominant megacolon mouse human homolog of MGC15649 PCWH SOX 10 SOX10 SOX10_HUMAN SRY (sex determining region Y) box 10 SRY (sex determining region Y) box 10 SRY box 10 SRY box containing gene 10 SRY related HMG box gene 10 SRY related HMG box gene 10 Transcription factor SOX 10 Transcription factor SOX-10 WS2E WS4 WS4C |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 1,000 (SOX10, HA601413, green); 1:2,000 (NeuN, ET1602-12, red) Antigen retrieval: Not required |
|
Fig2:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1: 1,000 |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-SOX10 antibody (HA601413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-SOX10 antibody (HA601413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.