MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric)
cat.: HA601414
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | IHC-Fr, IHC-P, WB, IF-Tissue |
| Clone number: | PSH02-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Immunogen: | Recombinant protein within human MAG aa 1-536 / 626. |
| Positive control: | Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Cell membrane, Membrane raft. |
| Recommended Dilutions:
IHC-Fr IHC-P WB IF-Tissue |
1:500-1:1,000 1:500 1:2,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P20916 Human | P20917 Mouse | P07722 Rat |
| Alternative names: | GMA MAG MAG_HUMAN Myelin associated glycoprotein Myelin-associated glycoprotein S MAG S-MAG Sialic acid binding Ig like lectin 4A Sialic acid binding immunoglobulin like lectin 4A Siglec 4a Siglec-4a SIGLEC4A SPG75 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required |
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Fig2:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Western blot analysis of MAG on different lysates with Rat anti-MAG antibody (HA601414) at 1/2,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 13 seconds; Lane 3: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.